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KMID : 0615720100270060031
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Jouranl of Korean Acupuncture and Moxibustion society
2010 Volume.27 No. 6 p.31 ~ p.42
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Anti-obesity Effect of Rhizoma Atractylodis Herbal Acupuncture in High Fat Diet-induced Obese ICR Mouse
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Youh Eun-Joo
Seo Byung-Kwan
Nam Sang-Su
Kang Sung-Keel
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Abstract
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Objectives:The aim of this study was to investigate the anti-obesity potential and mechanisms of action of Rhizoma Atractylodis(RA) herbal acupuncture in high fat diet- induced obese ICR mice.
Methods: Sample solutions for herbal acupuncture were prepared from the Rhizoma Atractylodis water extract powder at concentration of 150mg/kg and 300mg/kg with distilled water. Five week-old ICR mice acclimatized to the laboratory environment for 1 week were allocated into four groups: regular diet group (RD), high fat diet group(HFD), groups fed HFD with 150mg/kg RA herbal acupuncture treatment (RAE 150) and with 300mg/kg RA herbal acupuncture treatment(RAE 300). Herbal acupuncture groups were injected with either 150mg/kg or 300mg/kg of Rhizoma Atractylodis(RA) subcutaneously onto both Sinsu (BL23) alternately on the same time everyday for 30days. Body weight, gross appearance of epididymal fat area, blood glucose, insulin, insulin resistance(HOMA-IR), non-esterified fatty acid, cholesterol, triglyceride, AST, ALT, histological analysis of white adipose tissue, gene expression responsible for adipocyte differentiation and AMPK activation were analyzed.
Results: RA herbal acupuncture inhibited the development of weight gain, hyperglycemia, hyper- insulinemia, hyperlipidemia, increases of AST and ALT, and the enlargement of fat cell size induced by HFD. Also, RA herbal acupuncture inhibited the expression of PPAR-γ, C/EBPα, aP2, LPL, FAS, SCD-1 and enhanced the activation of AMP-activated protein kinase.
Conclusions: The results of this study demonstrate that RA herbal acupuncture can exert the anti-obesity effect and it is partially mediated by activation of AMPK and inhibition of the gene expressions responsible for adipocyte differentiation. Further studies will be required to ascertain the nti-obesity effect and mechanisms of action of RA herbal acupuncture in animal models and human for aclinical application.
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KEYWORD
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Rhizoma Atractylodis, herbal acupuncture, high fat diet, obesity, hyperglycemia, hyper- lipidemia, adipocyte differentiation, AMPK
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